AFB Test for Tuberculosis Detection | Sputum Test Guide

Learn how an AFB test diagnoses tuberculosis. Understand the sputum test process, benefits, and how results guide your treatment. Explore more here!

 

 

Category Details
What are AFB tests? AFB tests detect Acid-Fast Bacillus bacteria, causing tuberculosis (TB) and other mycobacterial infections like leprosy.
Types of AFB Tests AFB Smear: Sputum sample smeared on a slide and viewed under a microscope (results in 1-2 days).- AFB Culture: Sputum sent to lab to grow bacteria (results in 6-8 weeks).- Molecular Test (NAAT): Detects TB and resistance to rifampicin (results in less than 2 hours).- Susceptibility Test: Determines which antibiotics are most effective for treatment.
Purpose of AFB Tests – Diagnose active TB.- Identify other AFB infections (e.g., leprosy, Mycobacterium avium).- Monitor treatment progress for people already diagnosed with TB.
Symptoms of Active TB Pulmonary TB: Cough lasting 3+ weeks, chest pain, coughing up blood or sputum.- Other Symptoms: Chills, fever, night sweats, fatigue, unexplained weight loss, back pain (spinal TB), headache (TB meningitis), joint pain, abdominal pain.
Risk Factors – Recent TB infection.- Weakened immune system (e.g., HIV, diabetes, substance use, organ transplant, cancer treatments).- Contact with someone with TB.- Living/working in high-risk areas (e.g., homeless shelters, prisons).
Test Procedure Sample Collection: Cough into a sterile container to provide sputum. If needed, a saline mist or bronchoscopy (tube inserted into airways) may be used to collect sputum.
Preparation No special preparation required.
Risks – No risk from coughing sputum.- Minor risks from bronchoscopy: sore throat, small risk of infection or bleeding.
Test Results Negative Smear/Culture: Likely no active TB, or not enough bacteria to detect.- Positive Smear: Likely TB or other infection, needs culture confirmation.- Positive Culture: Confirms active TB or another AFB infection.- Positive NAAT: Confirms TB.- Susceptibility Test: Identifies effective antibiotics.
Treatment TB can be cured with antibiotics, but treatment takes 6-12 months. Follow the treatment plan fully to avoid relapse.
Important Notes TB can be deadly if untreated. After a few weeks of antibiotics, TB is no longer contagious, but you must complete the full course to cure it.

 

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